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Part:BBa_K4143337:Design

Designed by: Michigan Synthetic Biology Team   Group: iGEM22_Michigan   (2022-09-20)


T4GALA Encapsulin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Following purification of the encapsulin-AMP protein,, lowering the pH of the protein environment triggers disassembly of the encapsulin nanocompartment, allowing a TEV protease site to be accessed and ultimately the AMP is removed from the structure


Source

Encapsulins are bacterial organelle-like nanocompartments whose function is dictated by whatever its cargo is.

References